Raoultella ornithinolytica PX02 is a Gram-negative, encapsulated bacterium, part of the Enterobacteriaceae family, emerging as a notable human pathogen. Here, we present the whole genome sequence of R. ornithinolytica PX02 isolated from San Jacinto River sediment near a Burnet Shores community in Baytown, Texas. This microorganism harbors a large 200,000 bp incF plasmid and can potentially be a significant antibiotic reservoir. The PX02 genome consists of 5,970,914 base pairs encoding approximately 5,661 functional proteins. Strain PX02 (chromosomal and plasmid) was compiled at the scaffold level and can be accessed through the National Center for Biotechnology Information database under accession NZ_NJBC00000000.1.